magcellect cd44 high cd24 low breast cancer stem cell isolation kit Search Results


magcollect cd24 cd44 breast cancer stem cell isolation kit  (R&D Systems)
94
R&D Systems magcollect cd24 cd44 breast cancer stem cell isolation kit
Figure 2. RT‑R‑MDA‑MB‑231 cells demonstrate higher levels of CSCs marker <t>CD44,</t> but lower levels of <t>CD24</t> compared with MDA‑MB‑231 cells. Classical CSCs markers <t>CD44</t> and CD24 were detected in the low metastatic breast cancer cells MCF‑7 and RT‑R‑MCF‑7 (A) and highly metastatic breast cancer cells MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 (B) using the specific antibodies by western blotting. Data represent mean values ± standard error of the mean of three independent experiments in triplicate. **P<0.01 compared with control. CD, cluster of differentiation; CSCs, cancer stem cells; RT‑R, radioresistant.
Magcollect Cd24 Cd44 Breast Cancer Stem Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
magcollect cd24 cd44 breast cancer stem cell isolation kit - by Bioz Stars, 2026-03
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cd24 cd44 breast tumor stem cells  (R&D Systems)
93
R&D Systems cd24 cd44 breast tumor stem cells
Enumeration of TISC frequency in various murine passages following dietary salt modification. The frequency of <t>CD44+CD24−TISCs</t> cells in the isolated tumor cells among RS (grey) and HS (red) diet cohorts was enumerated by flow cytometry. ( A , B ) Representative flow cytometry plot of TISCs from passage 1 and passage 4 of Py230-C57Bl/6J HS diet cohort. ( C , D ) Changes in TISC frequency with each passage in Py230-C57Bl/6J ( C ) and 4T1-BALB/cJ ( D ) murine tumor models. ( E – N ) The mRNA expression of TISC markers Cadherin 1 ( E , J ), Snail2 ( F , K ), Aldh1A1 ( G , L ), Sox2 ( H , M ), and ITGA6 ( I , N ) in four passages of Py230-C57Bl/6J and 4T1-BALB/cJ tumor models (respectively). Data analyzed by one-way ANOVA for multiple comparisons and presented as mean ± SEM; n = 8 (biological replicates) per cohort; (*) p -value < 0.05.
Cd24 Cd44 Breast Tumor Stem Cells, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd24 cd44 breast tumor stem cells/product/R&D Systems
Average 93 stars, based on 1 article reviews
cd24 cd44 breast tumor stem cells - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


Figure 2. RT‑R‑MDA‑MB‑231 cells demonstrate higher levels of CSCs marker CD44, but lower levels of CD24 compared with MDA‑MB‑231 cells. Classical CSCs markers CD44 and CD24 were detected in the low metastatic breast cancer cells MCF‑7 and RT‑R‑MCF‑7 (A) and highly metastatic breast cancer cells MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 (B) using the specific antibodies by western blotting. Data represent mean values ± standard error of the mean of three independent experiments in triplicate. **P<0.01 compared with control. CD, cluster of differentiation; CSCs, cancer stem cells; RT‑R, radioresistant.

Journal: Oncology reports

Article Title: Radioresistant breast cancer cells exhibit increased resistance to chemotherapy and enhanced invasive properties due to cancer stem cells.

doi: 10.3892/or.2018.6714

Figure Lengend Snippet: Figure 2. RT‑R‑MDA‑MB‑231 cells demonstrate higher levels of CSCs marker CD44, but lower levels of CD24 compared with MDA‑MB‑231 cells. Classical CSCs markers CD44 and CD24 were detected in the low metastatic breast cancer cells MCF‑7 and RT‑R‑MCF‑7 (A) and highly metastatic breast cancer cells MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 (B) using the specific antibodies by western blotting. Data represent mean values ± standard error of the mean of three independent experiments in triplicate. **P<0.01 compared with control. CD, cluster of differentiation; CSCs, cancer stem cells; RT‑R, radioresistant.

Article Snippet: Isolation of CD24low/CD44high breast cancer stem cells was performed using a MagCollect CD24- CD44+ Breast Cancer Stem Cell Isolation kit (R&D Systems, Inc., Minneapolis, MN, USA) following the manufacturer's protocol.

Techniques: Marker, Western Blot, Control

Figure 3. RT‑R‑MDA‑MB‑231 cells increase the populations of CD24low/CD44high cells, and expression levels of other CSCs markers Notch‑4, Oct‑3/4 and ALDH1. (A) CD24low/CD44high cells were isolated from MDA‑MB‑231 and RT‑R‑MDA‑MB cells, and then the number of CD24low/CD44high breast cancer stem cells was quantified by flow cytometry. (B) MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 were labeled with anti‑CD24 and anti‑CD44 antibodies, and the percentages of CD24 or CD44‑expressed subpopulation in cells were determined by flow cytometry. (C) Other cancer stem cell markers, including Notch‑4, Oct3/4 and ALDH1 were detected in the MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 cells using the specific antibodies by western blotting. Data represent mean values ± standard error of the mean of three independent experiments in triplicate. *P<0.05 and **P<0.01 compared with MDA‑MB‑231 cells. ALDH1, aldehyde dehydrogenase 1; CD, cluster of differentiation; CSCs, cancer stem cells; Oct, octamer‑binding transcription factor; RT‑R, radioresistant.

Journal: Oncology reports

Article Title: Radioresistant breast cancer cells exhibit increased resistance to chemotherapy and enhanced invasive properties due to cancer stem cells.

doi: 10.3892/or.2018.6714

Figure Lengend Snippet: Figure 3. RT‑R‑MDA‑MB‑231 cells increase the populations of CD24low/CD44high cells, and expression levels of other CSCs markers Notch‑4, Oct‑3/4 and ALDH1. (A) CD24low/CD44high cells were isolated from MDA‑MB‑231 and RT‑R‑MDA‑MB cells, and then the number of CD24low/CD44high breast cancer stem cells was quantified by flow cytometry. (B) MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 were labeled with anti‑CD24 and anti‑CD44 antibodies, and the percentages of CD24 or CD44‑expressed subpopulation in cells were determined by flow cytometry. (C) Other cancer stem cell markers, including Notch‑4, Oct3/4 and ALDH1 were detected in the MDA‑MB‑231 and RT‑R‑MDA‑MB‑231 cells using the specific antibodies by western blotting. Data represent mean values ± standard error of the mean of three independent experiments in triplicate. *P<0.05 and **P<0.01 compared with MDA‑MB‑231 cells. ALDH1, aldehyde dehydrogenase 1; CD, cluster of differentiation; CSCs, cancer stem cells; Oct, octamer‑binding transcription factor; RT‑R, radioresistant.

Article Snippet: Isolation of CD24low/CD44high breast cancer stem cells was performed using a MagCollect CD24- CD44+ Breast Cancer Stem Cell Isolation kit (R&D Systems, Inc., Minneapolis, MN, USA) following the manufacturer's protocol.

Techniques: Expressing, Isolation, Flow Cytometry, Labeling, Western Blot

Enumeration of TISC frequency in various murine passages following dietary salt modification. The frequency of CD44+CD24−TISCs cells in the isolated tumor cells among RS (grey) and HS (red) diet cohorts was enumerated by flow cytometry. ( A , B ) Representative flow cytometry plot of TISCs from passage 1 and passage 4 of Py230-C57Bl/6J HS diet cohort. ( C , D ) Changes in TISC frequency with each passage in Py230-C57Bl/6J ( C ) and 4T1-BALB/cJ ( D ) murine tumor models. ( E – N ) The mRNA expression of TISC markers Cadherin 1 ( E , J ), Snail2 ( F , K ), Aldh1A1 ( G , L ), Sox2 ( H , M ), and ITGA6 ( I , N ) in four passages of Py230-C57Bl/6J and 4T1-BALB/cJ tumor models (respectively). Data analyzed by one-way ANOVA for multiple comparisons and presented as mean ± SEM; n = 8 (biological replicates) per cohort; (*) p -value < 0.05.

Journal: Cells

Article Title: Chronic High-Salt Diet Activates Tumor-Initiating Stem Cells Leading to Breast Cancer Proliferation

doi: 10.3390/cells13110912

Figure Lengend Snippet: Enumeration of TISC frequency in various murine passages following dietary salt modification. The frequency of CD44+CD24−TISCs cells in the isolated tumor cells among RS (grey) and HS (red) diet cohorts was enumerated by flow cytometry. ( A , B ) Representative flow cytometry plot of TISCs from passage 1 and passage 4 of Py230-C57Bl/6J HS diet cohort. ( C , D ) Changes in TISC frequency with each passage in Py230-C57Bl/6J ( C ) and 4T1-BALB/cJ ( D ) murine tumor models. ( E – N ) The mRNA expression of TISC markers Cadherin 1 ( E , J ), Snail2 ( F , K ), Aldh1A1 ( G , L ), Sox2 ( H , M ), and ITGA6 ( I , N ) in four passages of Py230-C57Bl/6J and 4T1-BALB/cJ tumor models (respectively). Data analyzed by one-way ANOVA for multiple comparisons and presented as mean ± SEM; n = 8 (biological replicates) per cohort; (*) p -value < 0.05.

Article Snippet: The TISCs were isolated from murine tumors by CD24 − CD44 + breast tumor stem cells using immunomagnetic beads as per the manufacturer’s specifications and reagents (catalog#MAGH111, R&D Systems, Minneapolis, MN, USA).

Techniques: Modification, Isolation, Flow Cytometry, Expressing

TGFβ signaling induced the expression of immune-inhibitory CD80 on TISCs. ( A ) Representative flow cytometry histogram of TGFβR2 expression in passages 1 (blue), 2 (pink), 3 (olive green), and 4 (purple) from the HS diet cohort of the Py230-C57Bl/6J tumor model. ( B ) Comparison of the relative expression of TGFβR2 in each of the four passages on CD4+CD24-TISCs isolated from RS (grey) and HS (red) diet cohorts of the Py230-C57Bl/6J tumor model. ( C ) Representative flow cytometry histogram of pSMAD2/3 intracellular expression in each of the four passages from the HS diet cohort of the Py230-C57Bl/6J tumor model. ( D ) Comparison of the relative expression of pSMAD2/3 in each of the four passages on CD4+CD24-TISCs isolated from the RS and HS diet cohorts of the Py230-C57Bl/6J tumor model. ( E ) Representative flow cytometry histogram of CD80 surface expression in each of the four passages from the HS diet cohort of the Py230-C57Bl/6J tumor model. ( F ) Comparison of the relative expression of CD80 in each of the four passages on CD4+CD24-TISCs isolated from the RS and HS diet cohorts of the Py230-C57Bl/6J tumor model. ( G ) Representative flow cytometry plot to determine the TGFβR2/CD80 double-positive TISCs in passage 4 of the HS diet cohort from the Py230-C57Bl/6J tumor model. ( H ) Comparison of the relative expression of TGFβR2/CD80 double-positive TISCs in each of the four passages on CD4+CD24-TISCs isolated from the RS and HS diet cohorts of the Py230-C57Bl/6J tumor model. ( I ) Comparison of the relative expression of CD80 following TGFβ (80 ng/mL) stimulation for 5 days on CD4+CD24-TISCs isolated from each of the four passages of the RS and HS diet cohorts in the Py230-C57Bl/6J tumor model. ( J – M ) Comparison of the relative expression of TGFβR2 ( J ), pSMAd2/3 ( K ), CD80 ( L ), and TGFβR2/CD80 double-positive cells ( M ) in each of the four passages on CD4+CD24-TISCs isolated from the RS (grey) and HS (red) diet cohorts of the 4T1-BALB/cJ tumor model. ( N ) Comparison of the relative expression of CD80 following TGFβ (80 ng/mL) treatment for 5 days on CD4+CD24-TISCs isolated from each of the four passages of the RS and HS diet cohorts of the 4T1-BALB/cJ tumor model. Data analyzed by one-way ANOVA for multiple comparisons and presented as mean ± SEM; n = 8 (biological replicates) per cohort; (*) p -value < 0.05.

Journal: Cells

Article Title: Chronic High-Salt Diet Activates Tumor-Initiating Stem Cells Leading to Breast Cancer Proliferation

doi: 10.3390/cells13110912

Figure Lengend Snippet: TGFβ signaling induced the expression of immune-inhibitory CD80 on TISCs. ( A ) Representative flow cytometry histogram of TGFβR2 expression in passages 1 (blue), 2 (pink), 3 (olive green), and 4 (purple) from the HS diet cohort of the Py230-C57Bl/6J tumor model. ( B ) Comparison of the relative expression of TGFβR2 in each of the four passages on CD4+CD24-TISCs isolated from RS (grey) and HS (red) diet cohorts of the Py230-C57Bl/6J tumor model. ( C ) Representative flow cytometry histogram of pSMAD2/3 intracellular expression in each of the four passages from the HS diet cohort of the Py230-C57Bl/6J tumor model. ( D ) Comparison of the relative expression of pSMAD2/3 in each of the four passages on CD4+CD24-TISCs isolated from the RS and HS diet cohorts of the Py230-C57Bl/6J tumor model. ( E ) Representative flow cytometry histogram of CD80 surface expression in each of the four passages from the HS diet cohort of the Py230-C57Bl/6J tumor model. ( F ) Comparison of the relative expression of CD80 in each of the four passages on CD4+CD24-TISCs isolated from the RS and HS diet cohorts of the Py230-C57Bl/6J tumor model. ( G ) Representative flow cytometry plot to determine the TGFβR2/CD80 double-positive TISCs in passage 4 of the HS diet cohort from the Py230-C57Bl/6J tumor model. ( H ) Comparison of the relative expression of TGFβR2/CD80 double-positive TISCs in each of the four passages on CD4+CD24-TISCs isolated from the RS and HS diet cohorts of the Py230-C57Bl/6J tumor model. ( I ) Comparison of the relative expression of CD80 following TGFβ (80 ng/mL) stimulation for 5 days on CD4+CD24-TISCs isolated from each of the four passages of the RS and HS diet cohorts in the Py230-C57Bl/6J tumor model. ( J – M ) Comparison of the relative expression of TGFβR2 ( J ), pSMAd2/3 ( K ), CD80 ( L ), and TGFβR2/CD80 double-positive cells ( M ) in each of the four passages on CD4+CD24-TISCs isolated from the RS (grey) and HS (red) diet cohorts of the 4T1-BALB/cJ tumor model. ( N ) Comparison of the relative expression of CD80 following TGFβ (80 ng/mL) treatment for 5 days on CD4+CD24-TISCs isolated from each of the four passages of the RS and HS diet cohorts of the 4T1-BALB/cJ tumor model. Data analyzed by one-way ANOVA for multiple comparisons and presented as mean ± SEM; n = 8 (biological replicates) per cohort; (*) p -value < 0.05.

Article Snippet: The TISCs were isolated from murine tumors by CD24 − CD44 + breast tumor stem cells using immunomagnetic beads as per the manufacturer’s specifications and reagents (catalog#MAGH111, R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Flow Cytometry, Comparison, Isolation